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December 31, 2015
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We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters.
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Simard, O., Leduc, F., Acteau, G., Arguin, M., Grégoire, M., Brazeau, M., Marois, I., Richter, M. V., Boissonneault, G. Step-specific Sorting of Mouse Spermatids by Flow Cytometry. J. Vis. Exp. (106), e53379, doi:10.3791/53379 (2015).
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