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Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
JoVE Journal
Medicine
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JoVE Journal Medicine
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

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14,471 Views

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09:29 min

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August 13, 2014

DOI:

10.3791/51419-v

DOI:
10.3791/51419-v

•

09:29 min
August 13, 2014

•

9 Views
, , , , ,
1Multi Organ Transplant Program,Toronto General Hospital, 2Department of Surgery,Toronto General Hospital, 3Department of Medicine,Toronto General Hospital

Summary

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Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.

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Knaak, J. M., Spetzler, V. N., Goldaracena, N., Louis, K. S., Selzner, N., Selzner, M. Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts. J. Vis. Exp. (90), e51419, doi:10.3791/51419 (2014).

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