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January 06, 2014
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Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).
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George, P., Sharma, A., Sharakhov, I. V. 2D and 3D Chromosome Painting in Malaria Mosquitoes. J. Vis. Exp. (83), e51173, doi:10.3791/51173 (2014).
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