Journal
/
Immunology and Infection
/
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

English

Automatically Generated

28,792 Views

•

08:31 min

•

October 15, 2013

DOI:

10.3791/50730-v

DOI:
10.3791/50730-v

•

08:31 min
October 15, 2013

•

8 Views
, , ,
1Department of Pediatrics, Centre for Understanding and Preventing Infection in Children,University of British Columbia

Summary

Automatically generated

Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.

Explore More Videos

Protein ExtractionBurkholderia Species2-D Gel ElectrophoresisProteomicsIsoelectric FocusingSDS-PAGESilver Staining

Read Article

Cite this Article

Velapatiño, B., Zlosnik, J. E. A., Hird, T. J., Speert, D. P. Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species. J. Vis. Exp. (80), e50730, doi:10.3791/50730 (2013).

Download .ris file

Copy

Share Video

.

Copy