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Immunology and Infection
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Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis

Live-cell Video Microscopy of Fungal Pathogen Phagocytosis

English

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38,408 Views

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08:52 min

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January 09, 2013

DOI:

10.3791/50196-v

DOI:
10.3791/50196-v

•

08:52 min
January 09, 2013

•

69 Views
, , , ,
1Division of Applied Medicine,University of Aberdeen, 2Aberdeen Fungal Group,University of Aberdeen

Summary

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We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.

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Cite this Article

Lewis, L. E., Bain, J. M., Okai, B., Gow, N. A., Erwig, L. P. Live-cell Video Microscopy of Fungal Pathogen Phagocytosis. J. Vis. Exp. (71), e50196, doi:10.3791/50196 (2013).

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