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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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April 23, 2012
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The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.
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QuantificationFungal ColonizationSporogenesisMycotoxinsKernel BioassaysSeed-infecting FungiCereal ProductionMaizePathogen-induced LossesGrain IntegrityMycotoxin Seed ContaminationAflatoxinFumonisinAspergillus FlavusFusarium VerticillioidesMolecular Plant-pathogen InteractionsStandardized MethodQuantifying Biological ParametersCross-interpretationKernel AssaysQuantitative AnalysesFungal GrowthBiomassMycotoxin Contamination
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Christensen, S., Borrego, E., Shim, W., Isakeit, T., Kolomiets, M. Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays. J. Vis. Exp. (62), e3727, doi:10.3791/3727 (2012).
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