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Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
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January 10, 2012
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A novel high-throughput method is described that enables the detection and relative quantitation of small RNA and mRNA expression from single bacterial cells using locked nucleic acid probes and flow cytometry-fluorescence in situ hybridization.
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Locked Nucleic AcidFlow CytometryFluorescence In Situ HybridizationFISHNucleic Acid SequencesFlow-FISHLysate-based Nucleic Acid Detection MethodsStatistical AnalysisVariance AnalysisTelomere Length DeterminationCellular IdentificationGene ExpressionViral MultiplicationBacterial Community AnalysisDNA Oligonucleotide ProbesNucleic Acid AnalogsSensitivitySpecificityMelting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes
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Robertson, K. L., Vora, G. J. Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection. J. Vis. Exp. (59), e3655, doi:10.3791/3655 (2012).
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