The goal of this article is to demonstrate a method to optimize immunofluorescent detection of estrogen receptor-α (ERα) in rat pial arterial slices using a digital immunofluorescent microscope.
Rezvani, N., Blokhin, A. V., Zeynalov, E., Littleton-Kearney, M. T. Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope. J. Vis. Exp. (57), e3203, doi:10.3791/3203 (2011).