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November 10, 2011
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The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
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Gopalakrishnan, S., Harris, E. N. In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. J. Vis. Exp. (57), e3138, doi:10.3791/3138 (2011).
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