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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
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May 01, 2011
DOI:
1Center for Biological Security,Robert Koch-Institute, 2CICVyA – INTA Castelar,Instituto de Virología, 3Center of Molecular Immunology,Ciudad de la Habana, Cuba, 4Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology,Free University of Berlin, 5Institut of Pharmacology,Charité-University Medicine of Berlin
Summary
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This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.
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IgY TechnologyChicken AntibodiesEgg YolkPolyethylene Glycol (PEG) PrecipitationImmunizationI.m. VaccinationGene-Gun Plasmid-immunizationAntibody-titresBoost ImmunizationsLaying CapacityExtraction Of Total IgYPrecipitation ProcedureRemoval Of LipidsDialysisIgY-extract StoragePurity Of The ExtractTotal IgY Per EggAge Of The Laying HenTotal Potential Harvest Of IgY
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Pauly, D., Chacana, P. A., Calzado, E. G., Brembs, B., Schade, R. IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation. J. Vis. Exp. (51), e3084, doi:10.3791/3084 (2011).
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