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April 01, 2011
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This article describes an in vitro technique for monitoring cancer cells invading through a monolayer of endothelial cells. The data is acquired in real-time as a function of changes in impedance on the surface of electrodes at the well bottom.
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Real-timeElectrical ImpedanceTechniqueMeasureInvasionEndothelial Cell MonolayerCancer CellsMetastatic DisseminationBasement Membrane DegradationTumor Cell AttachmentVascular EndotheliumEndothelial Junction RetractionInvasion And MigrationCirculatory SystemAdherence To Capillary WallsMetastatic Tumors FormationTumor Cell-endothelial Cell InteractionIn Vitro ReplicationMonolayer Of Human Umbilical Vein Endothelial Cells (HUVEC)Cancer Cell ChallengeElectron MicroscopyPhase-contrast MicroscopyIn Vivo Metastatic Process RepresentationElectrical Impedance Fluctuations MeasurementXCELLigence InstrumentRoche Manufacturing
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Rahim, S., Üren, A. A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells. J. Vis. Exp. (50), e2792, doi:10.3791/2792 (2011).
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