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Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
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May 10, 2011
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Here we describe a procedure allowing the labeling of Edwardsiella ictaluri in situ in histological sections from channel catfish Ictalurus punctatus using indirect immunohistochemistry with monoclonal antibodies Ed9 as a primary, and fluorescent FitC labeled antibodies as a secondary. This allowed for the detection of the bacterium using fluorescent microscopy.
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Fluorescent Immuno-ChemistryDetectionEdwardsiella IctaluriChannel CatfishIctalurus PunctatusMethod DevelopmentIn Situ VisualizationBacteriaHistological SectionsBacterial LocalizationPlate CountsRadiometric LabeledBioluminescent BacteriaGross Organ LevelInfection CycleVirulence FactorsInteraction With HostSalmonella TyphiTaxonomic FamilyTechnique DescriptionIndirect Immuno-histochemistryMonoclonal Ed9 AntibodyBlocking SerumParaffin Embedded Histological SectionsPrimary AntibodySecondary AntibodiesFitC Labeled Secondary Antibodies
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Menanteau-Ledouble, S., Lawrence, M. Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples. J. Vis. Exp. (51), e2687, doi:10.3791/2687 (2011).
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