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April 22, 2011
DOI:
1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine,Veterans Administration TVHS
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We describe the use of a mouse ES cell based assay to identify critical time windows for Wnt/β-catenin and BMP signal activation during cardiogenic induction. The method provides a standardized platform that reliably quantifies cardiogenic efficiency, and it is applicable to the study of other cell lineages.
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Modified Mouse Embryonic Stem Cell AssayQuantifying Cardiogenic Induction EfficiencyPluripotent Stem CellsKey Signaling PathwaysTemporal RegulationSpatial RegulationDifferentiation EfficiencyWnt/β-catenin SignalingBMP SignalingContracting Embryonic Bodies96-well Plate FormatCardiogenic EfficiencyModulator TreatmentsTime CourseCell LineagesHigh Throughput AssayAutomated Assay
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Ao, A., Williams, C. H., Hao, J., Hong, C. C. Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency. J. Vis. Exp. (50), e2656, doi:10.3791/2656 (2011).
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