Start with an adjuvant powder containing monophosphoryl lipid A or MPLA molecules.
This bacterial membrane-derived adjuvant enhances the vaccine's ability to activate immune cells.
Add a polar organic solvent. Briefly mix and incubate to dissolve the adjuvant.
In a separate container, mix the desired vaccine peptides with an optimal amount of a suitable emulsifier for improved dispersion upon contact with water.
Then, add squalene, an oil-based vaccine adjuvant.
While stirring gently, add the adjuvant solution to the mixture.
Add a large amount of water to the hydrophobic vaccine mixture to form an oil-in-water mixture.
The stirring process moves the vaccine components into the nano-sized droplet.
The emulsifier coats the droplet, preventing it from sticking to the other droplets.
This ensures the components are evenly dispersed for an ideal vaccine formulation.