Targeting Liver Tumors with Oncolytic Viruses via the Hepatic Artery
Transcript
Take an anesthetized rat containing a liver tumor.
Dissect the rat to expose the common hepatic artery that bifurcates into the proper hepatic artery, supplying blood to the liver and the gastroduodenal artery.
Ligate the gastroduodenal artery to prevent the virus from reaching other organs during subsequent injections.
Clamp the common hepatic artery to temporarily block the blood flow.
Inject oncolytic viruses into the gastroduodenal artery and ligate above the injection site to prevent bleeding.
Unclamp to allow the viruses to enter the proper hepatic artery and spread inside the liver. Close the incision and let the rat recover.
The oncolytic viruses enter the cells. Healthy cells produce functional antiviral proteins that block viral replication.
In cancer cells, the non-functional antiviral proteins fail to inhibit virus replication, ultimately resulting in cancer cell lysis.
