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Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

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13,905 Views

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13:36 min

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September 30, 2010

DOI:

10.3791/2168-v

DOI:
10.3791/2168-v

•

13:36 min
September 30, 2010

•

2 Views
, , , , ,
1Whitehead Institute for Biomedical Research, 2Departments of Microbiology and Biological Sciences,National University of Singapore, 3Department of Biology,Massachusetts Institute of Technology

Summary

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We demonstrate here that epigenetic reprogramming via Somatic Cell Nuclear Transfer (SCNT) can be used as a tool to generate mouse models with pre-defined T cell receptor (TCR) specificities. These transnuclear mice express the corresponding TCR from their endogenous locus under the control of the endogenous promoter.

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Kirak, O., Frickel, E., Grotenbreg, G. M., Suh, H., Jaenisch, R., Ploegh, H. L. Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT. J. Vis. Exp. (43), e2168, doi:10.3791/2168 (2010).

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