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In Vitro Mouse Bone Marrow Stromal Cell Differentiation into Adipocytes
Transcript
To induce bone-marrow stem-cell adipocyte differentiation, replace the cell-culture medium of a confluent bone marrow cell culture with adipocyte-induction medium. After two days of culture, replace the supernatant with fresh adipocyte-induction medium, switching to adipocyte-based medium on day 4. On day 7, confirm that cells have accumulated lipid droplets and that the display of phenotype is similar to mature adipocytes.
