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September 24, 2010
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We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.
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Rishal, I., Rozenbaum, M., Fainzilber, M. Axoplasm Isolation from Rat Sciatic Nerve. J. Vis. Exp. (43), e2087, doi:10.3791/2087 (2010).
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