Intra-uterine Cancer Cell Injection: A Technique to Deliver Cancer Cells for Establishing Intra-peritoneal Ovarian Cancer Mouse Model
Transcript
Intra-uterine cell injection allows the delivery of cells of interest into the uterine cavity of a recipient animal. To begin, prep an anesthetized immunocompromised nude mouse in the right lateral position. Make a surgical incision and dissect the abdominal muscle to expose its abdominal cavity.
Locate one of the uterine horns. These tubular structures are analogous to two distinct uteri, extending from the oviducts on both sides and opening into a single cervix.
Next, clamp the horn's anterior and posterior ends for segmentation. Now, slowly inject the ovarian cancer cell suspension into the segmented uterine lumen, ensuring efficient delivery of cells.
Subsequently, return the uterine horn into the abdominal cavity and suture the surgical incision. Allow the mouse to recover in a cage.
Within days of injection, tumors develop at the site of injection that metastasize to form secondary tumors in different tissues or organs in the peritoneal cavity.
