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Immunology and Infection
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Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems
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Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

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01:17 min

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January 05, 2024

DOI:

10.3791/201441-v

DOI:
10.3791/201441-v

•

01:17 min
January 05, 2024

•

1 Views
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1Department of Microbiology,University of Manitoba

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Electrocompetent CellsSingle-copy Gene ExpressionEfflux-deficient Bacterial StrainCell PreparationCentrifugationCell PelletSterile WaterCell SuspensionMicrofuge TubesElectroporation Cuvettes

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White, D., Kumar, A. Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain. J. Vis. Exp. (Pending Publication), e201441, doi:10.3791/201441 (2024).

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