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December 03, 2009
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The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.
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GELFREE 8100 Fractionation SystemProtein FractionationMolecular Weight-based FractionationLiquid Phase RecoverySingle-use CartridgesGELFREE Fractionation InstrumentConstant VoltageElectrophoretic MobilityResolving GelStacking GelCollection ChamberFractions CollectionPipetteComplex Protein MixturesTotal Protein Per Channel
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Witkowski, C., Harkins, J. Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery. J. Vis. Exp. (34), e1842, doi:10.3791/1842 (2009).
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