A subscription to JoVE is required to view this content. Sign in or start your free trial.
12,376 Views
•
12:24 min
•
March 05, 2010
DOI:
Summary
Automatically generated
In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
Explore More Videos
CD40-activationB CellsAntigen PresentationInterleukin-4CD40 LigandExpansionPeripheral BloodCD40-B CellsLymph Node Homing MoleculesT CellsAntigensMemory T CellsImmuno-stimulatory Antigen-presenting Cells (APC)Cell-based ImmunotherapyMurine CD40-activated B CellsSplenocytesPurified B CellsC57BL/6 MiceHaplotype H-2bDensity Gradient CentrifugationHeLa CellsRecombinant Murine CD40 Ligand (tmuCD40L HeLa)
Read Article
Cite this Article
Liebig, T. M., Fiedler, A., Klein-Gonzalez, N., Shimabukuro-Vornhagen, A., von Bergwelt-Baildon, M. Murine Model of CD40-activation of B cells. J. Vis. Exp. (37), e1734, doi:10.3791/1734 (2010).
Copy