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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

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20,765 Views

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11:56 min

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April 17, 2009

DOI:

10.3791/1205-v

DOI:
10.3791/1205-v

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11:56 min
April 17, 2009

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3 Views
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1Department of Evolutionary Functional Genomics, Evolutionary Biology Center,Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter,Swedish University of Agricultural Sciences

Summary

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We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.

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Karlgren, A., Carlsson, J., Gyllenstrand, N., Lagercrantz, U., Sundström, J. F. Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species. J. Vis. Exp. (26), e1205, doi:10.3791/1205 (2009).

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