Source: Otto, C. C. et al., Rapid Molecular Detection and Differentiation of Influenza Viruses A and B. J. Vis. Exp. (2017)
The video demonstrates an assay for quantifying influenza viruses A and B. This assay employs nicking endonuclease amplification with influenza-specific primers and target detection using molecular beacon probes.
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.
1. Before Running the Assay
NOTE: The Influenza A and B assay is approved for nasopharyngeal swab specimens and for nasopharyngeal swabs stored in viral transport media. Swabs are included in the kit and should be used for optimal performance. However, rayon, foam, flocked swabs, or polyester nasal swabs can also be used to collect nasal swab samples.
2. Running the Test
3. Quality Control (QC)
4. Result Interpretation
5. Maintenance and Cleaning
The authors have nothing to disclose.
Alere i Instrument | Alere | NAT-000 (Global), NAT-024 (US) | |
Alere i Influenza A & B 24 Test Kit | Alere | 425-000 (Global), 425-024 (US) | |
Alere i Barcode Scanner | Alere | EQ001001 | |
Alere Universal Printer | Alere | 55115 (Global), alereiprinter (US) | |
200 µL precision pipette | |||
200 µL disposable pipette tips | |||
Viral transport medium | Remel | M4-RT |