Source: John, S. et al., Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis. J. Vis. Exp. (2021)
This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Antigen preparation for subcutaneous injection
2. Subcutaneous injection
The authors have nothing to disclose.
Freund's Incomplete Adjuvant | BD Difco, NJ, USA | 263910 | |
H37Ra lyophilized Mycobacteria extract | BD Difco, NJ, USA | 231141 | |
Insulin needle | Exel International, USA | 26029 | 1 mL |
Isoflurane | |||
PBS | Gibco | 14190 | |
RM2255 | Leica Biosystems, IL,USA | Tissue Sectioning | |
TB Syringe | Becton, Dickinson and Company, NJ, USA | 309602 | 1 mL |
Tylenol | Johnson & Johnson Consumer Inc, PA, USA | NDC 50580-614-01 | Acetaminophen |