JOVE JOURNAL

Immunology and Infection

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A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

Effie E Bastounis¹, Fabian E Ortega¹, Ricardo Serrano², Julie A Theriot³

¹Department of Biochemistry,Stanford University School of Medicine, ²Department of Mechanical and Aerospace Engineering,University of California San Diego, ³Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute,Stanford University School of Medicine

Journal (Immunology and Infection)

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Volodymyr Tsvilovskyy¹, Alejandra Solis-Lopez¹, Kathrin Öhlenschläger¹, Marc Freichel¹

¹Institute of Pharmacology,Ruprecht-Karls Heidelberg University

Journal (Immunology and Infection)

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

Quinton L. Anderson¹, Alexey V. Revtovich¹, Natalia V. Kirienko¹

¹Department of Biosciences,Rice University

Journal (Immunology and Infection)

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Shouming Zhang^1,2,3, Xueping Gao^1,2,3, Genhui Xiao^1,2,3,4, Chengping Lu^1,2,3, Huochun Yao^1,2,3, Hongjie Fan^1,2,3,5, Zongfu Wu^1,2,3

¹College of Veterinary Medicine,Nanjing Agricultural University, ²Key Lab of Animal Bacteriology,Ministry of Agriculture, ³OIE Reference Lab for Swine Streptococcosis, ⁴School of Medicine,Tsinghua University, ⁵Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Journal (Immunology and Infection)

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Maiken W. Rosenstierne¹, Christopher E. Jensen¹, Anders Fomsgaard^1,2

¹Virus Research & Development Laboratory,Statens Serum Institut, ²Infectious Disease Research Unit,University of Southern Denmark

Journal (Immunology and Infection)

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Ryan M. Baxter*¹, Daniel S. Kong*¹, Josselyn E. Garcia-Perez¹, William E. O'Gorman², Elena W.Y. Hsieh^1,3

¹Department of Immunology and Microbiology,University of Colorado School of Medicine, ²OMNI Biomarkers, Development Sciences,Genentech, ³Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Journal (Immunology and Infection)

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

Jingshu Chen*², Lei Zhong*¹, Jing Wu¹, Sui Ke², Benjamin Morpurgo³, Andrei Golovko³, Nengtai Ouyang⁴, Yuxiang Sun², Shaodong Guo², Yanan Tian^1,2

¹Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,Hunan Agriculture University, ²Texas A&M University, ³Texas A&M Institute for Genomic Medicine, ⁴Sun Yat-Sen Memorial Hospital

Journal (Immunology and Infection)

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Ola H. Negm^1,2, Mohamed Hamed^1,2, Tanya M. Monaghan³

¹Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre,University of Nottingham, ²Medical Microbiology and Immunology Department, Faculty of Medicine,Mansoura University, ³Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre,University of Nottingham

Journal (Immunology and Infection)

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Jordan F. Hastings¹, Jeremy Z.R. Han¹, Robert F. Shearer^1,2, Sean P. Kennedy^1,3, Mary Iconomou^1,4, Darren N. Saunders⁵, David R. Croucher^1,6,7

¹The Kinghorn Cancer Centre,Garvan Institute of Medical Research, ²Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences,University of Copenhagen, ³RCSI Molecular Medicine,Royal College of Surgeons in Ireland, ⁴Department of Epigenetics,Max Planck Institute of Immunobiology and Epigenetics, ⁵School of Medical Sciences,University of New South Wales, ⁶St Vincent's Hospital Clinical School,University of New South Wales, ⁷School of Medicine and Medical Science,University College Dublin

Journal (Immunology and Infection)

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Meisam Naeimi Kararoudi¹, Hamid Dolatshad², Prashant Trikha¹, Syed-Rehan A. Hussain³, Ezgi Elmas¹, Jennifer A. Foltz¹, Jena E. Moseman¹, Aarohi Thakkar¹, Robin J. Nakkula¹, Margaret Lamb¹, Nitin Chakravarti¹, K. John McLaughlin³, Dean A. Lee¹

¹Center for Childhood Cancer and Blood Disease,Nationwide Children's Hospital, ²Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, ³Center for Clinical and Translational Research,Nationwide Children's Hospital Research Institute

Journal (Immunology and Infection)

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Helen Weavers^1,2, Anna Franz¹, Will Wood³, Paul Martin^1,4

¹School of Biochemistry, Biomedical Sciences,University of Bristol, ²School of Cellular and Molecular Medicine, Biomedical Sciences,University of Bristol, ³MRC Centre for Inflammation Research,University of Edinburgh, Queens Medical Research Institute, ⁴School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences,University of Bristol

Journal (Immunology and Infection)

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Alba Llibre*^1,2, Vincent Bondet*^1,2, Mathieu P. Rodero³, David Hunt⁴, Yanick J. Crow^3,5, Darragh Duffy^1,2

¹Immunobiology of Dendritic Cells,Institut Pasteur, ²INSERM U1223, ³Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163,Institut Imagine, ⁴MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine,University of Edinburgh, ⁵Manchester Centre for Genomic Medicine,University of Manchester

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