JOVE JOURNAL

Biology

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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Ho Lam Tang^1,2,3, Ho Man Tang^1,2,3, J. Marie Hardwick¹, Ming Chiu Fung²

¹W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, ²School of Life Sciences,Chinese University of Hong Kong, ³Center for Cell Dynamics, Department of Biological Chemistry,Johns Hopkins University School of Medicine

Journal (Biology)

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Petar N. Grozdanov¹, Clinton C. MacDonald¹

¹Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center

Journal (Biology)

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Louise Aigrain^1,2, Marko Sustarsic¹, Robert Crawford¹, Anne Plochowietz¹, Achillefs N. Kapanidis¹

¹Clarendon Laboratory, Department of Physics,University of Oxford, ²Wellcome Trust Sanger Institute,Genome Center

Journal (Biology)

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Alessandra Stefan^1,2, Alessandro Ceccarelli¹, Emanuele Conte¹, Alejandro Montón Silva¹, Alejandro Hochkoeppler^1,2

¹Department of Pharmacy and Biotechnology,University of Bologna, ²CSGI, Department of Chemistry,University of Firenze

Journal (Biology)

Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Christian Q. Scheckhuber¹

¹LOEWE Excellence Cluster for Integrative Fungal Research (IPF),Senckenberg Research Institute

Journal (Biology)

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

Matthias Garten¹, Sophie Aimon², Patricia Bassereau¹, Gilman E. S. Toombes³

¹Institut Curie, Centre de Recherche, CNRS, UMR 168, PhysicoChimie Curie,Université Pierre et Marie Curie, ²Kavli Institute for Brain and Mind,University of California, San Diego, ³Molecular Physiology and Biophysics Section, National Institute for Neurological Disorders and Stroke,National Institute of Health

Journal (Biology)

Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research

Brian Jespersen¹, Nathan R. Tykocki², Stephanie W. Watts¹, Peter J. Cobbett¹

¹Department of Pharmacology and Toxicology,Michigan State University, ²Department of Pharmacology,University of Vermont College of Medicine

Journal (Biology)

Isolation of Myofibroblasts from Mouse and Human Esophagus

Matthew Gargus¹, Chao Niu¹, Anisa Shaker¹

¹Department of Medicine,Keck School of Medicine, University of Southern California

Journal (Biology)

An Innovative Method for Exosome Quantification and Size Measurement

Arash Mehdiani¹, Anatol Maier¹, Antonio Pinto¹, Mareike Barth¹, Payam Akhyari¹, Artur Lichtenberg¹

¹Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty,Heinrich-Heine-University Dusseldorf

Journal (Biology)

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Atashi Sharma¹, Troy D. Anderson¹, Igor V. Sharakhov¹

¹Department of Entomology,Virginia Tech

Journal (Biology)

Do’s and Don’ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Vanessa Cabra¹, Montserrat Samsó¹

¹Department of Physiology and Biophysics,Virginia Commonwealth University

Journal (Biology)

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Carmen I. Nussbaum-Krammer¹, Mário F. Neto¹, Renée M. Brielmann¹, Jesper S. Pedersen¹, Richard I. Morimoto¹

¹Department of Molecular Biosciences, Rice Institute for Biomedical Research,Northwestern University

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