JOVE JOURNAL

Bioengineering

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Journal (Bioengineering)

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^1,2,4, Carina Henriques^1,2,5, Ana C. Silva^1,2,3, Diana D. Lobo^1,2,3, Luísa Cortes^1,2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC - Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC - Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC,University of Coimbra

Journal (Bioengineering)

Production of Recombinant AAV by Transient Transfection

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^1,2,4, Carina Henriques^1,2,5, Ana C. Silva^1,2,3, Diana D. Lobo^1,2,3, Luísa Cortes^1,2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC - Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC - Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC,University of Coimbra

Journal (Bioengineering)

FPLC Purification and Characterization of Recombinant AAV

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^1,2,4, Carina Henriques^1,2,5, Ana C. Silva^1,2,3, Diana D. Lobo^1,2,3, Luísa Cortes^1,2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC - Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC - Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC,University of Coimbra

Journal (Bioengineering)

Automated Compression Testing of the Ocular Lens

Debae Alzoubi¹, Wade Rich¹, Matthew A. Reilly^1,2

¹Department of Biomedical Engineering,The Ohio State University, ²Department of Ophthalmology & Visual Sciences,The Ohio State University

Journal (Bioengineering)

Isolation of Porcine Ocular Lens for Automated Compression Testing

Debae Alzoubi¹, Wade Rich¹, Matthew A. Reilly^1,2

¹Department of Biomedical Engineering,The Ohio State University, ²Department of Ophthalmology & Visual Sciences,The Ohio State University

Journal (Bioengineering)

Automated Compression Testing of Porcine Ocular Lens and Estimation of the Lens Modulus

Debae Alzoubi¹, Wade Rich¹, Matthew A. Reilly^1,2

¹Department of Biomedical Engineering,The Ohio State University, ²Department of Ophthalmology & Visual Sciences,The Ohio State University

Journal (Bioengineering)

Light-Sheet Imaging to Reveal Cardiac Structure in Rodent Hearts

Milad Almasian¹, Alireza Saberigarakani¹, Xinyuan Zhang¹, Brian Lee¹, Yichen Ding^1,2,3

¹Department of Bioengineering,The University of Texas at Dallas, ²Center for Imaging and Surgical Innovation,The University of Texas at Dallas, ³Hamon Center for Regenerative Science and Medicine,UT Southwestern Medical Center

Journal (Bioengineering)

Preparation of Rodent Heart Sample for Light-Sheet Microscopy

Milad Almasian¹, Alireza Saberigarakani¹, Xinyuan Zhang¹, Brian Lee¹, Yichen Ding^1,2,3

¹Department of Bioengineering,The University of Texas at Dallas, ²Center for Imaging and Surgical Innovation,The University of Texas at Dallas, ³Hamon Center for Regenerative Science and Medicine,UT Southwestern Medical Center

Journal (Bioengineering)

Light-Sheet Imaging of Rodent Hearts

Milad Almasian¹, Alireza Saberigarakani¹, Xinyuan Zhang¹, Brian Lee¹, Yichen Ding^1,2,3

¹Department of Bioengineering,The University of Texas at Dallas, ²Center for Imaging and Surgical Innovation,The University of Texas at Dallas, ³Hamon Center for Regenerative Science and Medicine,UT Southwestern Medical Center

Journal (Bioengineering)

Setting Up an Axially-Swept Light-Sheet System Hardware for Imaging Rodent Heart Samples

Milad Almasian¹, Alireza Saberigarakani¹, Xinyuan Zhang¹, Brian Lee¹, Yichen Ding^1,2,3

¹Department of Bioengineering,The University of Texas at Dallas, ²Center for Imaging and Surgical Innovation,The University of Texas at Dallas, ³Hamon Center for Regenerative Science and Medicine,UT Southwestern Medical Center

Journal (Bioengineering)

Isolation of Tendon Assembloid Components from Mice

Tino Stauber^1,2, Maja Wolleb^1,2, Jess G. Snedeker^1,2

¹Department of Orthopedics,Balgrist, University Hospital Zurich, University of Zurich, ²Department of Health Sciences and Technology, Institute for Biomechanics,ETH Zurich

Journal (Bioengineering)

Fabrication of Mouse Tendon Assembloids

Tino Stauber^1,2, Maja Wolleb^1,2, Jess G. Snedeker^1,2

¹Department of Orthopedics,Balgrist, University Hospital Zurich, University of Zurich, ²Department of Health Sciences and Technology, Institute for Biomechanics,ETH Zurich

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