JOVE JOURNAL

Biologia dello sviluppo

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Journal (Developmental Biology)

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Madison A. Rogers*¹, Brenna J. C. Dennison*¹, Katherine A. Fantauzzo¹

¹Department of Craniofacial Biology, School of Dental Medicine,University of Colorado Anschutz Medical Campus

Journal (Developmental Biology)

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots

Ines Rauschert*², Juan Claudio Benech*², Martha Sainz¹, Omar Borsani¹, Mariana Sotelo-Silveira¹

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República (UdelaR), ²Laboratorio de Señalización Celular y Nanobiología,Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)

Journal (Developmental Biology)

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Seoyoung Kang¹, Stefanie G. Wohl¹

¹Department of Biological and Vision Sciences, College of Optometry,The State University of New York

Journal (Developmental Biology)

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Lucile Boutaud*¹, Marie Michael*¹, Céline Banal², Damelys Calderon¹, Sarah Farcy¹, Julie Pernelle¹, Nicolas Goudin³, Camille Maillard¹, Clémantine Dimartino¹, Cécile Deleschaux¹, Sébastien Dupichaud⁴, Corinne Lebreton¹, Sophie Saunier¹, Tania Attié-Bitach^1,5, Nadia Bahi-Buisson^1,6, Nathalie Lefort², Sophie Thomas¹

¹Imagine Institute, INSERM UMR 1163,Université de Paris, ²Imagine Institute, iPSC Core Facility, INSERM UMR U1163,Université de Paris, ³Necker Bio-image Analysis platform of the SFR Necker, ⁴Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163,Université de Paris, ⁵Fédération de Génétique, Hôpital Necker-Enfants Malades,Assistance Publique Hôpitaux de Paris, ⁶Pediatric Neurology,APHP- Necker Enfants Malades Hospital

Journal (Developmental Biology)

Targeted Laser Ablation in the Embryo of Saccharina latissima

Samuel Boscq¹, Stéphanie Dutertre², Ioannis Theodorou¹, Bénédicte Charrier¹

¹UMR8227, CNRS / Sorbonne University, ²Univ Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, MRic Core Facility

Journal (Developmental Biology)

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Emma Laporte*¹, Charlotte Nys*¹, Hugo Vankelecom¹

¹Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven)

Journal (Developmental Biology)

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Katrin Mangold¹, Jingyan He¹, Sanne Stokman¹, Emma R. Andersson¹

¹Department of Cell and Molecular Biology,Karolinska Institutet

Journal (Developmental Biology)

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Olivia L. Hagen¹, Yehyun Kim¹, Elaine Kushkowski^1,2, Hannah Rouse³, Kara L. Cerveny¹

¹Department of Biology,Reed College, ²Committee on Development, Regeneration, and Stem Cell Biology,University of Chicago, ³Department of Cell and Developmental Biology,University College London

Journal (Developmental Biology)

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Yuki Shimizu^1,2, Takashi Kawasaki¹

¹Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology, ²DBT-AIST International Laboratory for Advanced Biomedicine,National Institute of Advanced Industrial Science and Technology

Journal (Developmental Biology)

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Maryam Aghadi^1,2, Manale Karam¹, Essam M. Abdelalim^1,2

¹Diabetes Research Center, Qatar Biomedical Research Institute (QBRI),Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), ²College of Health and Life Sciences,Hamad Bin Khalifa University (HBKU), Qatar Foundation

Journal (Developmental Biology)

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Jessica Cristina Marín-Llera¹, Jesús Chimal-Monroy¹

¹Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México

Journal (Developmental Biology)

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Jessica Cristina Marín-Llera¹, Montse Fernández-Calderón², Jesús Chimal-Monroy¹

¹Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, ²Departamento de Anatomía y Biología Celular and IDIVAL, Facultad de Medicina,Universidad de Cantabria

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