JOVE JOURNAL

Biochemistry

Showing 493 - 504 in 731 videos.

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Aleksandra Skrajna, Xiao-Cui Yang, Zbigniew Dominski²

¹Integrative Program for Biological and Genome Sciences,University of North Carolina at Chapel Hill, ²Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill

Journal (Biochemistry)

Studying Protein Import into Chloroplasts Using Protoplasts

Junho Lee, Hyangju Kang, Inhwan Hwang²

¹Division of Integrative Biosciences and Biotechnology,Pohang University of Science and Technology, ²Department of Life Sciences,Pohang University of Science and Technology

Journal (Biochemistry)

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Valentin Dunsing, Salvatore Chiantia

¹Institute for Biochemistry and Biology, Cell Membrane Biophysics Group,University of Potsdam

Journal (Biochemistry)

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Bidhan Chandra Nayak*¹, Jie Wang*¹, Lianyun Lin*^1,2,3,4, Weiyi He^3,4, Minsheng You^3,4, Zhiguang Yuchi²

¹Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology,Tianjin University, ²State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology,Fujian Agriculture and Forestry University, ³Joint International Research Laboratory of Ecological Pest Control,Ministry of Education, Fuzhou, ⁴Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests,Fujian Agriculture and Forestry University

Journal (Biochemistry)

Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy

Pradeep K. Singh*¹, Hanna E. Berk-Rauch*¹, Nadine Soplop, Kunihiro Uryu, Sidney Strickland, Hyung Jin Ahn

¹Patricia and John Rosenwald Laboratory of Neurobiology and Genetics,Rockefeller University, ²Electron Microscopy Resource Center,Rockefeller University

Journal (Biochemistry)

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Teresa Cordero, Verónica Aragonés, José-Antonio Daròs

¹Instituto de Biología Molecular y Celular de Plantas,Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain

Journal (Biochemistry)

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

Heidi A. Rossow, Gabriela Acetoze, John Champagne, Jon J. Ramsey

¹Veterinary Medicine Teaching and Research Center,University of California Davis, ²Archer Daniels Midland, ³Department of Molecular Biosciences,University of California Davis

Journal (Biochemistry)

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Hanna Axelsson², Helena Almqvist², Brinton Seashore-Ludlow³

¹Chemical Biology Consortium Sweden, Science for Life Laboratory,Karolinska Institutet, Solna, ²Department of Medical Biochemistry and Biophysics,Karolinska Institutet, Solna, ³Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet, Stockholm

Journal (Biochemistry)

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Javad Alizadeh², Shahla Shojaei^2,3, Simone da Silva Rosa, Adel Rezaei Moghadam², Amir A. Zeki, Mohammad Hashemi, Marek J. Los^7,8, Joseph W. Gordon⁹, Saeid Ghavami^2,10

¹Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine,University of Manitoba, ²Biology of Breathing Theme, Children Hospital Research Institute of Manitoba,University of Manitoba, ³Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences,Isfahan University of Medical Sciences, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine,Center for Comparative Respiratory Biology and Medicine, ⁵Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, ⁶Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec,Medical University of Silesia, ⁷Centre de biophysique moléculaire - UPR 4301,Centre national de la recherche scientifique (CNRS) CS80054, ⁸LinkoCare Life Sciences AB, ⁹College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences,University of Manitoba, ¹⁰Health Policy Research Center, Institute of Health,Shiraz University of Medical Sciences

Journal (Biochemistry)

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Tyler Mrozowich, Steffane McLennan, Michael Overduin⁴, Trushar R. Patel^3,4

¹Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry,University of Lethbridge, ²Department of Biochemistry,University of Alberta, ³Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary, ⁴DiscoveryLab, Faculty of Medicine & Dentistry,University of Alberta

Journal (Biochemistry)

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Graeden Winkelaar, Catharine Trieber, Jitendra Kumar, Michael Overduin

¹Department of Biochemistry,University of Alberta

Journal (Biochemistry)

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Smarajit Polley², De-Bin Huang, Tapan Biswas, Gourisankar Ghosh

¹Department of Chemistry & Biochemistry,University of California, San Diego, ²Department of Biophysics,Bose Institute

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