Encyclopedia Of Experiments

Caenorhabditis elegans (worm)

This collection features research techniques for the metazoan Caenorhabditis elegans. This nematode worm is a powerful model system due to its transparent body, defined developmental plan, robust genetic tools, and neuro-behavioral paradigms.

Encyclopedia Of Experiments

Caenorhabditis elegans (worm)

This collection features research techniques for the metazoan Caenorhabditis elegans. This nematode worm is a powerful model system due to its transparent body, defined developmental plan, robust genetic tools, and neuro-behavioral paradigms.

Sections

Basic Methods

Microscopy

Behavior

Anatomy and Physiology

Cell Biology

Genetics

Basic Methods

Microscopy

Behavior

Anatomy and Physiology

Cell Biology

Genetics

Basic Methods

Nematode Synchronization

A Method to Obtain Populations of Worms in Identical Stages of Development

Single Worm PCR

A Method to Extract and Amplify Genomic DNA

Lifespan Analysis

Measuring C. elegans Longevity

Egg Laying Assay

A Method to Quantify the Egg-Laying Behavior of C. elegans

Gonad Microinjection

A Method of Compound Delivery Directly into the Germline of C. elegans

Microscopy

Freeze-Cracking of Nematodes

A Method to Expose Interior Worm Tissues for Staining

Nematode Slide Preparation

A Method to Mount Animals on an Agar Pad

Calcium Imaging

A Method to Visualize Neural Activity in Live C. elegans

Behavior

Swimming-Induced Paralysis (SWIP) Assay

A Method to Quantify Dopamine-Mediated Locomotion in C. elegans

Touch Habituation Assay

A Method to Study Non-Associative Learning in C. elegans

C. elegans Chemotaxis Assay

A Method to Test Chemosensation in Worms

Electrotaxis Assay

A Method to Observe Locomotion in C. elegans

C. elegans Movement Tracking

A Method to Assess Locomotion in Worms

Anatomy and Physiology

C. elegans Gonad Extrusion

A Rapid Dissection Technique

C. elegans Survival Assay

A Short-Term Toxicity Test

C. elegans Muscle Area Measurements

A Standardized Method to Quantify Striated Muscle Morphology

Epidermal Laser Wounding

Inducing Localized Damage to Examine Repair Processes in C. elegans

FITC-Dextran Feeding

A Method to Quantify Intestinal Permeability in C. elegans

Cell Biology

Nile Red Staining of C. elegans

A Method to Visualize Lipid Droplets in Fixed Animals

Single Cell Dissociation of Caenorhabditis elegans

A Method to Isolate Live Cells

Ethanol Fixation and DAPI Staining

A Method to Visualize DNA in C. elegans

C. elegans Blastomere Dissection

A Method to Remove the Eggshell and Dissociate Embryonic Cells

Enzymatic Digestion and Manual Dissociation

A Method to Prepare C. elegans Embryos for Cell Culture

Genetics

RNAi Plating for C. elegans Feeding

A Technique to Induce Target dsRNA Expression in E. coli

RNAi Feeding in Liquid Culture

A High-Throughput Method to Knockdown Gene Expression in C. elegans

Enriching for Males

Maintaining a Stock of Male C. elegans

UV Irradiation

A Method to Integrate Extrachromosomal Arrays