JOVE JOURNAL

生物化学

Showing 517 - 528 in 776 videos.

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Journal (Biochemistry)

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Nicholas McCaul^1,2, Hui Ying Yeoh¹, Guus van Zadelhoff¹, Naomi Lodder¹, Bertrand Kleizen¹, Ineke Braakman¹

¹Cellular Protein Chemistry,Utrecht University, ²Program in Cellular and Molecular Medicine,Boston Children's Hospital

Journal (Biochemistry)

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Daniel Bruce¹, Emily Cardew¹, Stefanie Freitag-Pohl², Ehmke Pohl^1,2

¹Department of Biosciences,Durham University, ²Department of Chemistry,Durham University

Journal (Biochemistry)

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Christophe Jung¹, Max Schnepf¹, Peter Bandilla¹, Ulrich Unnerstall¹, Ulrike Gaul¹

¹Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM),Ludwig-Maximilians-Universität München

Journal (Biochemistry)

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Patrick Rosendahl Andreassen¹, Jens Sivkær Pettersen¹, Mikkel Jørgensen¹

¹Department of Biochemistry and Molecular Biology,University of Southern Denmark

Journal (Biochemistry)

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Micah P. Stumme-Diers¹, Tommy Stormberg¹, Zhiqiang Sun¹, Yuri L. Lyubchenko¹

¹Department of Pharmaceutical Sciences,University of Nebraska Medical Center

Journal (Biochemistry)

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

K. Martin Chow¹, Sidney W. Whiteheart¹, Jeffrey R. Smiley², Savita Sharma¹, Kathy Boaz², Meggie J. Coleman², Alvina Maynard³, Louis B. Hersh¹, Craig W. Vander Kooi¹

¹Department of Molecular and Cellular Biochemistry,University of Kentucky, ²Division of Laboratory Animal Resources,University of Kentucky, ³River Hill Ranch

Journal (Biochemistry)

Isolation of F₁-ATPase from the Parasitic Protist Trypanosoma brucei

Ondřej Gahura¹, Alena Zíková^1,2

¹Biology Centre,Czech Academy of Science, Institute of Parasitology, ²Faculty of Science,University of South Bohemia

Journal (Biochemistry)

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

William D. McCaig¹, Matthew A. Deragon¹, Robert J. Haluska Jr,¹, Alexa L. Hodges¹, Payal S. Patel¹, Timothy J. LaRocca¹

¹Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Journal (Biochemistry)

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Eva Königshausen¹, Sebastian A. Potthoff¹, Raphael Haase¹, Catherine Meyer-Schwesinger², Ernest Kaufmann¹, L. Christian Rump¹, Johannes Stegbauer¹, Lorenz Sellin¹, Ivo Quack¹, Magdalena Woznowski¹

¹Department of Nephrology, Medical Faculty,Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology,University Medical Center Hamburg-Eppendorf

Journal (Biochemistry)

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Beáta Bozóki*^1,2, János András Mótyán*¹, Márió Miczi¹, Lívia Diána Gazda¹, József Tőzsér¹

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine,University of Debrecen, ²Biotechnology Research Department,Gedeon Richter Plc

Journal (Biochemistry)

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Hanan B. Elajaili¹, Laura Hernandez-Lagunas¹, Kalina Ranguelova², Sergey Dikalov³, Eva Nozik-Grayck¹

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics,University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center

Journal (Biochemistry)

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Javier A. Jaimes*^1,2, Jean K. Millet*^1,3, Monty E. Goldstein^1,4, Gary R. Whittaker¹, Marco R. Straus¹

¹Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, ²Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, ³Virologie et Immunologie Moléculaires, Domaine de Vilvert,INRA, ⁴Department of Cell Biology and Molecular Genetics,University of Maryland

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