1Prion Research Center,Colorado State University, 2Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences,Colorado State University, 3Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences,Colorado State University
Adipose-derived mesenchymal stromal cells (AdMSCs) have potent immunomodulatory properties useful for treating diseases associated with inflammation. We demonstrate how to isolate and culture murine AdMSCs and primary mixed glia, stimulate AdMSCs to upregulate anti-inflammatory genes and growth factors, assess migration of AdMSCs, and co-culture AdMSCs with primary mixed prion-infected glia.
