Embryonic Mouse Brain Mixed Cell Cultures to Study Infection and Innate Immunity

Embryonic Mouse Brain Mixed Cell Cultures to Study Infection and Innate Immunity

June 30, 2023

Author Spotlight: Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity

Murine E17 Embryonal Brain Dissection, Cell Plating, and Maintenance

Alistair Gamble¹, Maria Suessmilch¹, Anniek Bonestroo¹, Andres Merits², Gerard J. Graham¹, Jonathan Cavanagh¹, Julia M. Edgar¹, Marieke Pingen¹

¹School of Infection and Immunity,University of Glasgow, ²Faculty of Science and Technology, Institute of Technology,University of Tartu

Summary

This protocol presents a unique way of generating central nervous system cell cultures from embryonic day 17 mouse brains for neuro(immuno)logy research. This model can be analyzed using various experimental techniques, including RT-qPCR, microscopy, ELISA, and flow cytometry.

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