ジャーナル
/
生物学
/
Visualization of Organelles In Situ by Cryo-STEM Tomography
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Visualization of Organelles In Situ by Cryo-STEM Tomography

Visualization of Organelles In Situ by Cryo-STEM Tomography

1,499 Views

•

08:37 min

•

June 23, 2023

DOI:

10.3791/65052-v

DOI:
10.3791/65052-v

•

08:37 min
June 23, 2023

•

9 Views
, , ,
1Department of Chemical and Biological Physics,Weizmann Institute of Sciences, 2Department of Chemical Research Support,Weizmann Institute of Sciences

概要

Automatically generated

Cryo-STEM tomography provides a means to visualize organelles of intact cells without embedding, sectioning, or other invasive preparations. The 3D resolution obtained is currently in the range of a few nanometers, with a field of view of several micrometers and an accessible thickness in the order of 1 µm.

Explore More Videos

Cryo-STEM TomographyTEM TomographyFIB SEMSoft X-ray TomographySTEM OpticsColumn AlignmentEucentric FocusSTEM ModeCondenser ApertureBeam ShiftRotation CenterPivot PointsCondenser Stigmators

Read Article

Cite this Article

Kirchweger, P., Mullick, D., Wolf, S. G., Elbaum, M. Visualization of Organelles In Situ by Cryo-STEM Tomography. J. Vis. Exp. (196), e65052, doi:10.3791/65052 (2023).

Download .ris file

Copy

Share Video

.

Copy