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Detección multiparamétrica de fármacos organoides tumorales mediante imágenes de células vivas de campo amplio para análisis a granel y de un solo organoide
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON),University of Antwerp, 2Industrial Vision Lab,University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery,University Hospital Antwerp (UZA), 4Department of Oncology,University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT),University of Antwerp
章
- 00:05Introduction
- 00:53Day 0: Preparation of the 2- or 3-Day-Old Organoids
- 02:55Days 2 – 3: Harvesting the 2- or 3-Day-Old Organoids
- 04:16Seeding the 2- or 3-Day-Old Organoids Using a Pipetting Robot
- 05:27Day 4: Drug Treatment and Reagent Dispensing with Digital Drug Dispenser
- 07:49Image Acquisition with the Live-Cell Imager and Analysis of Data
- 10:12Results: The Single-Organoid Dose-Response Analysis
- 12:03Conclusion
Este protocolo describe un método semiautomatizado para exámenes de detección de fármacos con organoides de rendimiento medio a alto y software de análisis de imágenes automatizado e independiente del microscopio para cuantificar y visualizar las respuestas multiparamétricas a fármacos de un solo organoide para capturar la heterogeneidad intratumoral.
タグ
MultiparametricTumor OrganoidDrug ScreeningLive-cell ImagingBulk AnalysisSingle-organoid AnalysisStreamlined WorkflowAutomated AnalysisClinically RelevantPatient-derived Tumor OrganoidsPDTOExtracellular MatrixECMDissociationCell SuspensionPDAC OrganoidCulture MediumCell PlatingECM DomesY-27632Organoid Harvesting
