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Multiparametrische tumor organoïde drug screening met behulp van Widefield Live-Cell Imaging voor bulk en single-organoïde analyse
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12:41 min
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December 23, 2022
DOI:
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON),University of Antwerp, 2Industrial Vision Lab,University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery,University Hospital Antwerp (UZA), 4Department of Oncology,University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT),University of Antwerp
概要
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Dit protocol beschrijft een semi-geautomatiseerde methode voor medium- tot high-throughput organoïde medicijnscreenings en microscoop-agnostische, geautomatiseerde beeldanalysesoftware om multiparametrische, single-organoïde geneesmiddelresponsen te kwantificeren en te visualiseren om intratumor heterogeniteit vast te leggen.
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MultiparametricTumor OrganoidDrug ScreeningLive-cell ImagingBulk AnalysisSingle-organoid AnalysisStreamlined WorkflowAutomated AnalysisClinically RelevantPatient-derived Tumor OrganoidsPDTOExtracellular MatrixECMDissociationCell SuspensionPDAC OrganoidCulture MediumCell PlatingECM DomesY-27632Organoid Harvesting
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Cite this Article
Le Compte, M., Cardenas De La Hoz, E., Peeters, S., Smits, E., Lardon, F., Roeyen, G., Vanlanduit, S., Prenen, H., Peeters, M., Lin, A., Deben, C. Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis. J. Vis. Exp. (190), e64434, doi:10.3791/64434 (2022).
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