Engineered flaps require an incorporated functional vascular network. In this protocol, we present a method of fabricating a 3D printed tissue flap containing a hierarchical vascular network and its direct microsurgical anastomoses to rat femoral artery.
Machour, M., Szklanny, A. A., Levenberg, S. Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies. J. Vis. Exp. (183), e63920, doi:10.3791/63920 (2022).