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免疫肽组学：分离小鼠和人MHC I类和II类相关肽用于质谱分析
JoVE Journal
生化学
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JoVE Journal 生化学
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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免疫肽组学：分离小鼠和人MHC I类和II类相关肽用于质谱分析

8,742 Views

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09:32 min

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October 15, 2021

DOI:

10.3791/63052-v

DOI:
10.3791/63052-v

•

09:32 min
October 15, 2021

•

77 Views
, , , ,
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine,Université de Montréal

概要

Automatically generated

在这里，我们提出了一种从小鼠和人细胞系纯化MHC I类和II类肽复合物的方案，提供高质量的免疫肽组学数据。该协议侧重于使用市售抗体进行样品制备。

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Cite this Article

Sirois, I., Isabelle, M., Duquette, J. D., Saab, F., Caron, E. Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis. J. Vis. Exp. (176), e63052, doi:10.3791/63052 (2021).

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