ジャーナル
/
発生生物学
/
Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

DOI:
10.3791/62818-v

•

05:28 min

•

August 19, 2021

•

,
1Department of Molecular Cell Biology,University of California

章

  • 00:05Introduction
  • 00:45Global Light Induction
  • 01:26Quantitative Real-time PCR and Fluorescence Microscopy
  • 03:59Results: Light-activated Gene Expression Assessment in Zebrafish Embryo
  • 04:46Conclusion

概要

自動翻訳

自動翻訳

Optogenetics is a powerful tool with wide-ranging applications. This protocol demonstrates how to achieve light-inducible gene expression in zebrafish embryos using the blue light-responsive TAEL/C120 system.

タグ

Light-induced GFP ExpressionOptogeneticTAEL/C120 SystemZebrafish EmbryosInducible Gene ExpressionLineage TracingGain-of-function AssaysRescue ExperimentsRNA ExtractionCDNA SynthesisQuantitative PCR

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Mechanical Vessel Injury in Zebrafish Embryos

Functional Cloning Using a Xenopus Oocyte Expression System

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Read Article