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基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线
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免疫学と感染
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JoVE Journal 免疫学と感染
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
Please note that all translations are automatically generated. Click here for the English version.

基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线

9,830 Views

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11:32 min

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November 13, 2021

DOI:

10.3791/62328-v

DOI:
10.3791/62328-v

•

11:32 min
November 13, 2021

•

30 Views
, , , , , , , , ,
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine,Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy,Aix Marseille Université

概要

Automatically generated

该协议使用荧光记者和细胞分拣来简化巨噬细胞和T细胞系的敲击实验。两个质粒用于这些简化的敲击实验，即CRISPR/Cas9-和DsRed2表达质粒和同源重组供体质粒表达EBFP2，这是永久整合在 Rosa26 点在免疫细胞。

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Cite this Article

Zhang, L., Huang, R., Lu, L., Fu, R., Guo, G., Gu, Y., Liu, Z., He, L., Malissen, M., Liang, Y. Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines. J. Vis. Exp. (177), e62328, doi:10.3791/62328 (2021).

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