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In vitro kwantitatieve beeldvormingstest voor fagocytose van dode neuroblastoomcellen door iPSC-macrofagen
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神経科学
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JoVE Journal 神経科学
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
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In vitro kwantitatieve beeldvormingstest voor fagocytose van dode neuroblastoomcellen door iPSC-macrofagen

5,009 Views

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09:48 min

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February 14, 2021

DOI:

10.3791/62217-v

DOI:
10.3791/62217-v

•

09:48 min
February 14, 2021

•

10 Views
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1James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, 3UK Dementia Research Institute,Cardiff University

概要

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Neurodegeneratieve ziekten worden geassocieerd met ontregelde microgliafuncties. Dit artikel schetst een in vitro test van fagocytose van neuroblastoomcellen door iPSC-macrofagen. Kwantitatieve microscopie-uitleingen worden beschreven voor zowel live-cell time-lapse imaging als fixed-cell high-content imaging.

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Cite this Article

Hall-Roberts, H., Di Daniel, E., James, W. S., Davis, J. B., Cowley, S. A. In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages. J. Vis. Exp. (168), e62217, doi:10.3791/62217 (2021).

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