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Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging
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発生生物学
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JoVE Journal 発生生物学
Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

DOI:
10.3791/62157-v

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09:20 min

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April 10, 2021

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,
1生化学・分子生物学科,Uniformed Services University

章

  • 00:00Introduction
  • 01:14Preparing Single Ovarioles for Live Imaging
  • 05:34H2O2 Treatment and Live Imaging
  • 06:37Results: Representative Changes in Mitochondrial and Clu Bliss Particle Subcellular Localization After H2O2 Addition
  • 08:25Conclusion

概要

自動翻訳

自動翻訳

The objective of this protocol is to use live imaging to visualize the effects of oxidative damage on the localization and dynamics of subcellular structures in Drosophila ovaries.

タグ

DrosophilaOxidative DamageLive ImagingMitochondriaRibonucleoprotein CluSchneider’s Drosophila MediaFetal Bovine SerumPen-StrepInsulinFemale Germ CellsFollicle DevelopmentWet Yeast Paste

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