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Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model
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JoVE Journal 免疫学と感染
Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

8,422 Views

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09:11 min

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February 14, 2021

DOI:

10.3791/62135-v

DOI:
10.3791/62135-v

•

09:11 min
February 14, 2021

•

13 Views
, , , , ,
1病理学科,University of Michigan, 2Department of Surgery, Klinikum Rechts der Isar,Technische Universität München, 3Wallenberg Centre for Molecular Medicine,Linköping University, 4Department of Biomedical and Clinical Sciences (BKV),Linköping University

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Systematic scoring of intestinal inflammation using a free computer-assisted system is a powerful tool to quantitatively compare histopathological changes in colitis models characterized by the presence of ulcers and inflammatory changes. Histological colitis score evaluation strengthens clinical observations and facilitates data interpretation.

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Keyword Extraction:Systematic Scoring AnalysisIntestinal InflammationMurine Dextran Sodium Sulfate-induced Colitis ModelMucosal InjuryHistological InterpretationQuantitative AnalysisIntestinesMiceColitisEuthanized MouseDissecting PadAbdominal SkinPeritoneumLarge IntestinePelvic BoneColonPBSWestern BlotPCRImmunofluorescence MicroscopyNeutral Buffered Formalin Solution

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Cite this Article

Garcia-Hernandez, V., Neumann, P., Koch, S., Lyons, R., Nusrat, A., Parkos, C. A. Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model. J. Vis. Exp. (168), e62135, doi:10.3791/62135 (2021).

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