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水生甲壳虫中的RNA干扰是特定发育时间点操纵基因表达的有力工具
JoVE Journal
環境学
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JoVE Journal 環境学
RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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水生甲壳虫中的RNA干扰是特定发育时间点操纵基因表达的有力工具

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08:55 min

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May 29, 2020

DOI:

10.3791/61477-v

DOI:
10.3791/61477-v

•

08:55 min
May 29, 2020

•

8 Views
, , , , ,
1Department of Biological Sciences,University of Cincinnati, 2生物学科,Miami University, 3生物学科,University of North Carolina at Chapel Hill, 4神経科学科,The Scripps Research Institute

概要

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RNA干扰是一种广泛适用、强大的技术，用于在特定发育阶段操纵基因表达。在这里，我们描述了在水生潜水甲虫热力学马莫拉图斯中实施这项技术的必要 步骤，从基因序列的获取到影响结构或行为的基因的敲击。

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Cite this Article

Rathore, S., Hassert, J., Clark-Hachtel, C. M., Stahl, A., Tomoyasu, Y., Bushbeck, E. K. RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points. J. Vis. Exp. (159), e61477, doi:10.3791/61477 (2020).

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