このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus
章
- 00:05Introduction
- 01:13Craniotomy and Animal Preparation
- 02:16AAV Cocktail Injection
- 03:08In Vivo Neural Recordings with Optogenetics
- 05:20Amplifier and Filter Settings
- 07:23Results: VTA Glutamate Projections to the Hippocampus Modulate CA1 Activity
- 08:42Conclusion
The current protocol demonstrates a simple method for tracing of ventral tegmental area (VTA) glutamate projections to the hippocampus. Photostimulation of VTA glutamate neurons was combined with CA1 recording to demonstrate how VTA glutamate terminals modulate CA1 putative pyramidal firing rate in vivo.
