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Isolamento delle cellule endocartali e coronarie dal muro libero ventricolare del cuore di ratto
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center
章
- 00:04Introduction
- 01:11Heart Isolation
- 02:34Endocardial Endothelial Cell (EEC) Digestion
- 03:34Coronary Endothelial Cell (CEC) Digestion
- 04:36Endothelial Cell (EC) Sorting
- 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
- 07:46Conclusion
Vi presentiamo un protocollo per l'isolamento delle cellule endocardiali e coronariche dai cuori dei ratti attraverso la digestione sequenziale dei tessuti in un buffer di digestione, la raccolta cellulare da cicli di centrifuga ricorrenti e la purificazione cellulare utilizzando microsfere CD31 anti-rat.
