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Isolierung von Endokardialen und koronaren Endothelzellen aus der ventrikulären freien Wand des Rattenherzes
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center
章
- 00:04Introduction
- 01:11Heart Isolation
- 02:34Endocardial Endothelial Cell (EEC) Digestion
- 03:34Coronary Endothelial Cell (CEC) Digestion
- 04:36Endothelial Cell (EC) Sorting
- 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
- 07:46Conclusion
