Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Fernando Gonz&#225;lez Ibanez; Katherine Picard; Maude Bordeleau; Kaushik Sharma; Kanchan Bisht; Marie-&#200;ve Tremblay

doi:10.3791/60510

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Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

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Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

29,283 Views

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10:40 min

•

October 27, 2019

DOI:

10.3791/60510-v

DOI:

10.3791/60510-v

•

10:40 min

October 27, 2019

•

119 Views

Fernando González Ibanez^1,2, Katherine Picard^1,2, Maude Bordeleau^1,3, Kaushik Sharma^1,2,4, Kanchan Bisht^1,2,4, Marie-Ève Tremblay^1,2

¹Axe Neurosciences,Centre de Recherche du CHU de Québec-Université Laval, ²Département de Médecine Moléculaire, Faculté de Médecine,Université Laval, ³Integrated Program in Neuroscience,McGill University, ⁴Center for Brain Immunology and Glia (BIG),University of Virginia

概要

This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue.

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Immunofluorescence IBA1 TMEM119 Microglia Peripheral Myeloid Cell Density Morphology Infiltration Mouse Brain Fiji ImageJ Nearest Neighbor Distance Scale Composite Image Channel Tool Freehand Selection Selection Brush Region Of Interest

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Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

概要

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