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Isolamento e Cultura de Adipócitos Maduros Humanos Usando culturas agregadas de adipócitomaduros de membrana (MAAC)
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生物学
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JoVE Journal 生物学
Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

Isolamento e Cultura de Adipócitos Maduros Humanos Usando culturas agregadas de adipócitomaduros de membrana (MAAC)

17,875 Views

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06:28 min

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February 13, 2020

DOI:

10.3791/60485-v

DOI:
10.3791/60485-v

•

06:28 min
February 13, 2020

•

37 Views
, ,
1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca, 2The Lundberg Laboratory for Diabetes Research,University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine,University of Gothenburg

概要

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A cultura agregada madura de membrana (MAAC) é um novo método para cultivar adipócitos humanos maduros. Aqui detalhamos como isolar adipócitos do adipose humano e como configurar o MAAC.

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Cite this Article

Alexandersson, I., Harms, M. J., Boucher, J. Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC). J. Vis. Exp. (156), e60485, doi:10.3791/60485 (2020).

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